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How do the results above help me choose the best tool for my data and objectives? #20

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Juke34 opened this issue Jan 23, 2025 · 3 comments
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@Juke34
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Juke34 commented Jan 23, 2025

No description provided.

Juke34 added a commit that referenced this issue Jan 24, 2025
…istics table, add section in readme to explain how to look at the results
@Juke34
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Juke34 commented Jan 24, 2025

I don't go into detail about the selection criteria for choosing the best tool, as this depends on the underlying biological issues, but I do calculate general statistics with FastQC. You should use the --fastqc to generate statistics.
I added a section in the README to go more into detail about what QC is available and where to find them.
I guess when I will add the description of how the outputs are structured (#19), this will become also clearer.

Is it enough to answer your concerns @rcannood ?

Juke34 added a commit that referenced this issue Jan 27, 2025
#20 provide statistics information in readme
Juke34 added a commit that referenced this issue Jan 28, 2025
#20 add citationt to point to documentation about daisy-chaining
Juke34 added a commit that referenced this issue Jan 28, 2025
#20 add samtools stats and provide more information about statistics
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Juke34 commented Jan 28, 2025

I have now also added samtools stats to provide more comprehensive statistics. Everything is collected into a single report with MultiQC that can help to compare the aligners.
I do not want to go into details on which statistics to focus on to select an aligner. It depends a lot on the downstream task.
I have added a sentence in the manuscript to tell what statistics is available :

To facilitate aligner comparison, AliNe provides a comprehensive set of metrics, including computational resource usage (CPU, memory, runtime, and disk I/O) reported by Nextflow, as well as alignment statistics generated by FastQC [@fastqc] and Samtools stats [@samtools].

I hope these changes address your concerns.

@rcannood
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rcannood commented Feb 3, 2025

Thanks @Juke34 ! The information in the readme helps with being able to interpret the results :) Feel free to close this issue!

@Juke34 Juke34 closed this as completed Feb 3, 2025
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