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From memory we had a previous issue with lowercase bases in the input, if so then it has returned
The attached test.gtf has the following splice sites
NC_037283.1:200919-201095(-) gt ag
NC_037283.1:201282-201445(-) GT ag
NC_037283.1:201512-201775(-) gt ag
NC_037283.1:203421-203569(-) gt ag
i.e. all are canonical
however running mikado prepare on this gtf and attached NC_037283.1.fa file results in no transcripts in the prepare gtf, rerunning with --lenient and this model is output marked as having no canonical introns.
If the NC_037283.1.fa file is converted to uppercase then prepare functions correctly i.e. all introns are regarded as canonical so the issue is with having lowercase bases (commonly used for softmasking)
From memory we had a previous issue with lowercase bases in the input, if so then it has returned
The attached test.gtf has the following splice sites
NC_037283.1:200919-201095(-) gt ag
NC_037283.1:201282-201445(-) GT ag
NC_037283.1:201512-201775(-) gt ag
NC_037283.1:203421-203569(-) gt ag
i.e. all are canonical
however running mikado prepare on this gtf and attached NC_037283.1.fa file results in no transcripts in the prepare gtf, rerunning with --lenient and this model is output marked as having no canonical introns.
If the NC_037283.1.fa file is converted to uppercase then prepare functions correctly i.e. all introns are regarded as canonical so the issue is with having lowercase bases (commonly used for softmasking)
test.gtf.zip
NC_037283.1.fa.zip
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