diff --git a/protocol-designer/src/assets/localization/en/feature_flags.json b/protocol-designer/src/assets/localization/en/feature_flags.json
index 92a074088ba..851f8deb5a9 100644
--- a/protocol-designer/src/assets/localization/en/feature_flags.json
+++ b/protocol-designer/src/assets/localization/en/feature_flags.json
@@ -12,10 +12,6 @@
     "title": "Allow all tip rack options",
     "description": "Enable selection of all tip racks for each pipette."
   },
-  "OT_PD_ENABLE_ABSORBANCE_READER": {
-    "title": "Enable absorbance plate reader",
-    "description": "Enable absorbance plate reader support."
-  },
   "OT_PD_ENABLE_COMMENT": {
     "title": "Enable comment step",
     "description": "You can add comments anywhere between timeline steps."
diff --git a/protocol-designer/src/feature-flags/reducers.ts b/protocol-designer/src/feature-flags/reducers.ts
index 336014c6691..efec2f863dd 100644
--- a/protocol-designer/src/feature-flags/reducers.ts
+++ b/protocol-designer/src/feature-flags/reducers.ts
@@ -23,8 +23,6 @@ const initialFlags: Flags = {
     process.env.OT_PD_DISABLE_MODULE_RESTRICTIONS === '1' || false,
   OT_PD_ALLOW_ALL_TIPRACKS:
     process.env.OT_PD_ALLOW_ALL_TIPRACKS === '1' || false,
-  OT_PD_ENABLE_ABSORBANCE_READER:
-    process.env.OT_PD_ENABLE_ABSORBANCE_READER === '1' || false,
   OT_PD_ENABLE_COMMENT: process.env.OT_PD_ENABLE_COMMENT === '1' || false,
   OT_PD_ENABLE_RETURN_TIP: process.env.OT_PD_ENABLE_RETURN_TIP === '1' || false,
   OT_PD_ENABLE_HOT_KEYS_DISPLAY:
diff --git a/protocol-designer/src/feature-flags/selectors.ts b/protocol-designer/src/feature-flags/selectors.ts
index 6b8a70f8b30..d673ad6bf09 100644
--- a/protocol-designer/src/feature-flags/selectors.ts
+++ b/protocol-designer/src/feature-flags/selectors.ts
@@ -25,10 +25,6 @@ export const getAllowAllTipracks: Selector<boolean> = createSelector(
   getFeatureFlagData,
   flags => flags.OT_PD_ALLOW_ALL_TIPRACKS ?? false
 )
-export const getEnableAbsorbanceReader: Selector<boolean> = createSelector(
-  getFeatureFlagData,
-  flags => flags.OT_PD_ENABLE_ABSORBANCE_READER ?? false
-)
 export const getEnableComment: Selector<boolean> = createSelector(
   getFeatureFlagData,
   flags => flags.OT_PD_ENABLE_COMMENT ?? false
diff --git a/protocol-designer/src/feature-flags/types.ts b/protocol-designer/src/feature-flags/types.ts
index 6840786d149..53ea6615c8b 100644
--- a/protocol-designer/src/feature-flags/types.ts
+++ b/protocol-designer/src/feature-flags/types.ts
@@ -25,13 +25,13 @@ export const DEPRECATED_FLAGS = [
   'OT_PD_ENABLE_MULTI_TIP',
   'OT_PD_ENABLE_MOAM',
   'OT_PD_ENABLE_REDESIGN',
+  'OT_PD_ENABLE_ABSORBANCE_READER',
 ]
 // union of feature flag string constant IDs
 export type FlagTypes =
   | 'PRERELEASE_MODE'
   | 'OT_PD_DISABLE_MODULE_RESTRICTIONS'
   | 'OT_PD_ALLOW_ALL_TIPRACKS'
-  | 'OT_PD_ENABLE_ABSORBANCE_READER'
   | 'OT_PD_ENABLE_COMMENT'
   | 'OT_PD_ENABLE_RETURN_TIP'
   | 'OT_PD_ENABLE_HOT_KEYS_DISPLAY'
@@ -46,7 +46,6 @@ export const userFacingFlags: FlagTypes[] = [
 export const allFlags: FlagTypes[] = [
   ...userFacingFlags,
   'PRERELEASE_MODE',
-  'OT_PD_ENABLE_ABSORBANCE_READER',
   'OT_PD_ENABLE_COMMENT',
   'OT_PD_ENABLE_RETURN_TIP',
   'OT_PD_ENABLE_REACT_SCAN',
diff --git a/protocol-designer/src/pages/CreateNewProtocolWizard/SelectModules.tsx b/protocol-designer/src/pages/CreateNewProtocolWizard/SelectModules.tsx
index 19e757ee0dc..717a6b2b762 100644
--- a/protocol-designer/src/pages/CreateNewProtocolWizard/SelectModules.tsx
+++ b/protocol-designer/src/pages/CreateNewProtocolWizard/SelectModules.tsx
@@ -1,5 +1,4 @@
 import { useTranslation } from 'react-i18next'
-import { useSelector } from 'react-redux'
 import {
   ALIGN_CENTER,
   BORDERS,
@@ -25,7 +24,6 @@ import {
   TEMPERATURE_MODULE_TYPE,
 } from '@opentrons/shared-data'
 import { uuid } from '../../utils'
-import { getEnableAbsorbanceReader } from '../../feature-flags/selectors'
 import { useKitchen } from '../../organisms/Kitchen/hooks'
 import { ModuleDiagram } from './ModuleDiagram'
 import { WizardBody } from './WizardBody'
@@ -51,7 +49,6 @@ export function SelectModules(props: WizardTileProps): JSX.Element | null {
   const fields = watch('fields')
   const modules = watch('modules')
   const additionalEquipment = watch('additionalEquipment')
-  const enableAbsorbanceReader = useSelector(getEnableAbsorbanceReader)
   const robotType = fields.robotType
   const supportedModules =
     robotType === FLEX_ROBOT_TYPE
@@ -166,8 +163,7 @@ export function SelectModules(props: WizardTileProps): JSX.Element | null {
       >
         <Flex flexDirection={DIRECTION_COLUMN}>
           <Flex flexDirection={DIRECTION_COLUMN} gridGap={SPACING.spacing12}>
-            {(filteredSupportedModules.length > 0 && enableAbsorbanceReader) ||
-            // note (kk:09/26/2024) the condition for absorbanceReaderV1 will be removed when ff is removed
+            {filteredSupportedModules.length > 0 ||
             !(
               filteredSupportedModules.length === 1 &&
               filteredSupportedModules[0] === 'absorbanceReaderV1'
@@ -179,11 +175,6 @@ export function SelectModules(props: WizardTileProps): JSX.Element | null {
             <Flex gridGap={SPACING.spacing4} flexWrap={WRAP}>
               {filteredSupportedModules
                 .sort((moduleA, moduleB) => moduleA.localeCompare(moduleB))
-                .filter(module =>
-                  enableAbsorbanceReader
-                    ? module
-                    : module !== ABSORBANCE_READER_V1
-                )
                 .map(moduleModel => {
                   const numSlotsAvailable = getNumSlotsAvailable(
                     modules,
diff --git a/protocol-designer/src/pages/CreateNewProtocolWizard/__tests__/SelectModules.test.tsx b/protocol-designer/src/pages/CreateNewProtocolWizard/__tests__/SelectModules.test.tsx
index c8c0a07cf7b..2d23f3a1fef 100644
--- a/protocol-designer/src/pages/CreateNewProtocolWizard/__tests__/SelectModules.test.tsx
+++ b/protocol-designer/src/pages/CreateNewProtocolWizard/__tests__/SelectModules.test.tsx
@@ -3,7 +3,6 @@ import '@testing-library/jest-dom/vitest'
 import { FLEX_ROBOT_TYPE, OT2_ROBOT_TYPE } from '@opentrons/shared-data'
 import { fireEvent, screen } from '@testing-library/react'
 import { i18n } from '../../../assets/localization'
-import { getEnableAbsorbanceReader } from '../../../feature-flags/selectors'
 import { renderWithProviders } from '../../../__testing-utils__'
 import { SelectModules } from '../SelectModules'
 
@@ -44,7 +43,6 @@ describe('SelectModules', () => {
     props = {
       ...mockWizardTileProps,
     } as WizardTileProps
-    vi.mocked(getEnableAbsorbanceReader).mockReturnValue(true)
   })
   afterEach(() => {
     vi.restoreAllMocks()
diff --git a/protocol-designer/src/pages/Designer/DeckSetup/DeckSetupTools.tsx b/protocol-designer/src/pages/Designer/DeckSetup/DeckSetupTools.tsx
index 51ceab2e937..d997835e831 100644
--- a/protocol-designer/src/pages/Designer/DeckSetup/DeckSetupTools.tsx
+++ b/protocol-designer/src/pages/Designer/DeckSetup/DeckSetupTools.tsx
@@ -49,7 +49,6 @@ import {
   selectNestedLabware,
   selectZoomedIntoSlot,
 } from '../../../labware-ingred/actions'
-import { getEnableAbsorbanceReader } from '../../../feature-flags/selectors'
 import { useBlockingHint } from '../../../organisms/BlockingHintModal/useBlockingHint'
 import { selectors } from '../../../labware-ingred/selectors'
 import { useKitchen } from '../../../organisms/Kitchen/hooks'
@@ -110,7 +109,6 @@ export function DeckSetupTools(props: DeckSetupToolsProps): JSX.Element | null {
     'change_magnet_module_model'
   )
   const dispatch = useDispatch<ThunkDispatch<any>>()
-  const enableAbsorbanceReader = useSelector(getEnableAbsorbanceReader)
   const deckSetup = useSelector(getDeckSetupForActiveItem)
   const {
     selectedLabwareDefUri,
@@ -144,9 +142,7 @@ export function DeckSetupTools(props: DeckSetupToolsProps): JSX.Element | null {
   }, [selectedModuleModel, selectedFixture])
 
   const moduleModels =
-    slot != null
-      ? getModuleModelsBySlot(enableAbsorbanceReader, robotType, slot)
-      : null
+    slot != null ? getModuleModelsBySlot(robotType, slot) : null
   const [tab, setTab] = useState<'hardware' | 'labware'>(
     moduleModels?.length === 0 || slot === 'offDeck' ? 'labware' : 'hardware'
   )
diff --git a/protocol-designer/src/pages/Designer/DeckSetup/LabwareTools.tsx b/protocol-designer/src/pages/Designer/DeckSetup/LabwareTools.tsx
index 0daab2f845e..df6c982a132 100644
--- a/protocol-designer/src/pages/Designer/DeckSetup/LabwareTools.tsx
+++ b/protocol-designer/src/pages/Designer/DeckSetup/LabwareTools.tsx
@@ -49,7 +49,6 @@ import {
   selectLabware,
   selectNestedLabware,
 } from '../../../labware-ingred/actions'
-import { getEnableAbsorbanceReader } from '../../../feature-flags/selectors'
 import {
   ALL_ORDERED_CATEGORIES,
   CUSTOM_CATEGORY,
@@ -134,8 +133,6 @@ export function LabwareTools(props: LabwareToolsProps): JSX.Element {
     robotType === OT2_ROBOT_TYPE ? isNextToHeaterShaker : false
   )
 
-  const enablePlateReader = useSelector(getEnableAbsorbanceReader)
-
   const getLabwareCompatible = useCallback(
     (def: LabwareDefinition2) => {
       // assume that custom (non-standard) labware is (potentially) compatible
@@ -171,8 +168,7 @@ export function LabwareTools(props: LabwareToolsProps): JSX.Element {
           moduleType !== HEATERSHAKER_MODULE_TYPE) ||
         (isAdapter96Channel && !has96Channel) ||
         (slot === 'offDeck' && isAdapter) ||
-        (!enablePlateReader &&
-          PLATE_READER_LOADNAME === parameters.loadName &&
+        (PLATE_READER_LOADNAME === parameters.loadName &&
           moduleType !== ABSORBANCE_READER_TYPE)
       )
     },
diff --git a/protocol-designer/src/pages/Designer/DeckSetup/__tests__/DeckSetupTools.test.tsx b/protocol-designer/src/pages/Designer/DeckSetup/__tests__/DeckSetupTools.test.tsx
index 0283b9ab724..cfc366baf85 100644
--- a/protocol-designer/src/pages/Designer/DeckSetup/__tests__/DeckSetupTools.test.tsx
+++ b/protocol-designer/src/pages/Designer/DeckSetup/__tests__/DeckSetupTools.test.tsx
@@ -17,7 +17,6 @@ import {
   getSavedStepForms,
 } from '../../../../step-forms/selectors'
 import { getRobotType } from '../../../../file-data/selectors'
-import { getEnableAbsorbanceReader } from '../../../../feature-flags/selectors'
 import { deleteDeckFixture } from '../../../../step-forms/actions/additionalItems'
 import { selectors } from '../../../../labware-ingred/selectors'
 import { getDismissedHints } from '../../../../tutorial/selectors'
@@ -68,7 +67,6 @@ describe('DeckSetupTools', () => {
     })
     vi.mocked(LabwareTools).mockReturnValue(<div>mock labware tools</div>)
     vi.mocked(getRobotType).mockReturnValue(FLEX_ROBOT_TYPE)
-    vi.mocked(getEnableAbsorbanceReader).mockReturnValue(true)
     vi.mocked(getDeckSetupForActiveItem).mockReturnValue({
       labware: {},
       modules: {},
diff --git a/protocol-designer/src/pages/Designer/DeckSetup/__tests__/utils.test.ts b/protocol-designer/src/pages/Designer/DeckSetup/__tests__/utils.test.ts
index 7d48c7ea71d..c2925185923 100644
--- a/protocol-designer/src/pages/Designer/DeckSetup/__tests__/utils.test.ts
+++ b/protocol-designer/src/pages/Designer/DeckSetup/__tests__/utils.test.ts
@@ -16,10 +16,10 @@ import { FLEX_MODULE_MODELS, OT2_MODULE_MODELS } from '../constants'
 
 describe('getModuleModelsBySlot', () => {
   it('renders no modules for ot-2 middle slot', () => {
-    expect(getModuleModelsBySlot(false, OT2_ROBOT_TYPE, '5')).toEqual([])
+    expect(getModuleModelsBySlot(OT2_ROBOT_TYPE, '5')).toEqual([])
   })
   it('renders all ot-2 modules for slot 7', () => {
-    expect(getModuleModelsBySlot(false, OT2_ROBOT_TYPE, '7')).toEqual(
+    expect(getModuleModelsBySlot(OT2_ROBOT_TYPE, '7')).toEqual(
       OT2_MODULE_MODELS
     )
   })
@@ -28,7 +28,7 @@ describe('getModuleModelsBySlot', () => {
       model =>
         model !== THERMOCYCLER_MODULE_V1 && model !== THERMOCYCLER_MODULE_V2
     )
-    expect(getModuleModelsBySlot(false, OT2_ROBOT_TYPE, '1')).toEqual(noTC)
+    expect(getModuleModelsBySlot(OT2_ROBOT_TYPE, '1')).toEqual(noTC)
   })
   it('renders ot-2 modules minus thermocyclers & heater-shaker for slot 9', () => {
     const noTCAndHS = OT2_MODULE_MODELS.filter(
@@ -37,15 +37,15 @@ describe('getModuleModelsBySlot', () => {
         model !== THERMOCYCLER_MODULE_V2 &&
         model !== HEATERSHAKER_MODULE_V1
     )
-    expect(getModuleModelsBySlot(false, OT2_ROBOT_TYPE, '9')).toEqual(noTCAndHS)
+    expect(getModuleModelsBySlot(OT2_ROBOT_TYPE, '9')).toEqual(noTCAndHS)
   })
   it('renders flex modules for middle slots', () => {
-    expect(getModuleModelsBySlot(false, FLEX_ROBOT_TYPE, 'B2')).toEqual([
+    expect(getModuleModelsBySlot(FLEX_ROBOT_TYPE, 'B2')).toEqual([
       MAGNETIC_BLOCK_V1,
     ])
   })
   it('renders all flex modules for B1', () => {
-    expect(getModuleModelsBySlot(true, FLEX_ROBOT_TYPE, 'B1')).toEqual(
+    expect(getModuleModelsBySlot(FLEX_ROBOT_TYPE, 'B1')).toEqual(
       FLEX_MODULE_MODELS.filter(model => model !== ABSORBANCE_READER_V1)
     )
   })
@@ -54,7 +54,7 @@ describe('getModuleModelsBySlot', () => {
       model =>
         model !== THERMOCYCLER_MODULE_V2 && model !== ABSORBANCE_READER_V1
     )
-    expect(getModuleModelsBySlot(true, FLEX_ROBOT_TYPE, 'C1')).toEqual(noTC)
+    expect(getModuleModelsBySlot(FLEX_ROBOT_TYPE, 'C1')).toEqual(noTC)
   })
 })
 
diff --git a/protocol-designer/src/pages/Designer/DeckSetup/utils.ts b/protocol-designer/src/pages/Designer/DeckSetup/utils.ts
index 0b1a455f3d4..1da3609df68 100644
--- a/protocol-designer/src/pages/Designer/DeckSetup/utils.ts
+++ b/protocol-designer/src/pages/Designer/DeckSetup/utils.ts
@@ -61,7 +61,6 @@ export function getCutoutIdForAddressableArea(
 }
 
 export function getModuleModelsBySlot(
-  enableAbsorbanceReader: boolean,
   robotType: RobotType,
   slot: DeckSlotId
 ): ModuleModelExtended[] {
@@ -88,7 +87,7 @@ export function getModuleModelsBySlot(
             if (model === THERMOCYCLER_MODULE_V2) {
               return slot === 'B1'
             } else if (model === ABSORBANCE_READER_V1) {
-              return FLEX_RIGHT_SLOTS.has(slot) && enableAbsorbanceReader
+              return FLEX_RIGHT_SLOTS.has(slot)
             } else if (
               model === TEMPERATURE_MODULE_V2 ||
               model === HEATERSHAKER_MODULE_V1
diff --git a/protocol-designer/src/pages/Designer/ProtocolSteps/Timeline/AddStepButton.tsx b/protocol-designer/src/pages/Designer/ProtocolSteps/Timeline/AddStepButton.tsx
index 52460aee461..16d7add611e 100644
--- a/protocol-designer/src/pages/Designer/ProtocolSteps/Timeline/AddStepButton.tsx
+++ b/protocol-designer/src/pages/Designer/ProtocolSteps/Timeline/AddStepButton.tsx
@@ -45,10 +45,7 @@ import {
   ConfirmDeleteModal,
   getMainPagePortalEl,
 } from '../../../../organisms'
-import {
-  getEnableAbsorbanceReader,
-  getEnableComment,
-} from '../../../../feature-flags/selectors'
+import { getEnableComment } from '../../../../feature-flags/selectors'
 import { AddStepOverflowButton } from './AddStepOverflowButton'
 
 import type { MouseEvent } from 'react'
@@ -87,7 +84,6 @@ export function AddStepButton({ hasText }: AddStepButtonProps): JSX.Element {
   const [enqueuedStepType, setEnqueuedStepType] = useState<StepType | null>(
     null
   )
-  const enableAbsorbanceReader = useSelector(getEnableAbsorbanceReader)
 
   const getSupportedSteps = (): Array<
     Exclude<StepType, 'manualIntervention'>
@@ -116,9 +112,7 @@ export function AddStepButton({ hasText }: AddStepButtonProps): JSX.Element {
     temperature: getIsModuleOnDeck(modules, TEMPERATURE_MODULE_TYPE),
     thermocycler: getIsModuleOnDeck(modules, THERMOCYCLER_MODULE_TYPE),
     heaterShaker: getIsModuleOnDeck(modules, HEATERSHAKER_MODULE_TYPE),
-    absorbanceReader:
-      getIsModuleOnDeck(modules, ABSORBANCE_READER_TYPE) &&
-      enableAbsorbanceReader,
+    absorbanceReader: getIsModuleOnDeck(modules, ABSORBANCE_READER_TYPE),
   }
 
   const addStep = (stepType: StepType): ReturnType<any> =>
diff --git a/protocol-designer/src/step-forms/selectors/index.ts b/protocol-designer/src/step-forms/selectors/index.ts
index 3dcac3bd735..160410217e4 100644
--- a/protocol-designer/src/step-forms/selectors/index.ts
+++ b/protocol-designer/src/step-forms/selectors/index.ts
@@ -675,15 +675,13 @@ export const getInvariantContext: Selector<
   getAdditionalEquipmentEntities,
   featureFlagSelectors.getDisableModuleRestrictions,
   featureFlagSelectors.getAllowAllTipracks,
-  featureFlagSelectors.getEnableAbsorbanceReader,
   (
     labwareEntities,
     moduleEntities,
     pipetteEntities,
     additionalEquipmentEntities,
     disableModuleRestrictions,
-    allowAllTipracks,
-    enableAbsorbanceReader
+    allowAllTipracks
   ) => ({
     labwareEntities,
     moduleEntities,
@@ -692,7 +690,6 @@ export const getInvariantContext: Selector<
     config: {
       OT_PD_ALLOW_ALL_TIPRACKS: Boolean(allowAllTipracks),
       OT_PD_DISABLE_MODULE_RESTRICTIONS: Boolean(disableModuleRestrictions),
-      OT_PD_ENABLE_ABSORBANCE_READER: Boolean(enableAbsorbanceReader),
     },
   })
 )