diff --git a/pandas/tests/io/excel/test_readers.py b/pandas/tests/io/excel/test_readers.py
index 431a50477fccc..05d8d5e43dd98 100644
--- a/pandas/tests/io/excel/test_readers.py
+++ b/pandas/tests/io/excel/test_readers.py
@@ -606,6 +606,7 @@ def test_read_from_http_url(self, read_ext):
         tm.assert_frame_equal(url_table, local_table)
 
     @td.skip_if_not_us_locale
+    @pytest.mark.single
     def test_read_from_s3_url(self, read_ext, s3_resource, s3so):
         # Bucket "pandas-test" created in tests/io/conftest.py
         with open("test1" + read_ext, "rb") as f:
diff --git a/pandas/tests/io/json/test_pandas.py b/pandas/tests/io/json/test_pandas.py
index 64a666079876f..c7e70e9768888 100644
--- a/pandas/tests/io/json/test_pandas.py
+++ b/pandas/tests/io/json/test_pandas.py
@@ -1213,6 +1213,7 @@ def test_read_inline_jsonl(self):
         tm.assert_frame_equal(result, expected)
 
     @td.skip_if_not_us_locale
+    @pytest.mark.single
     def test_read_s3_jsonl(self, s3_resource, s3so):
         # GH17200
 
@@ -1702,6 +1703,7 @@ def test_json_multiindex(self, dataframe, expected):
         result = series.to_json(orient="index")
         assert result == expected
 
+    @pytest.mark.single
     def test_to_s3(self, s3_resource):
         import time
 
diff --git a/pandas/tests/io/parser/test_network.py b/pandas/tests/io/parser/test_network.py
index b8b03cbd14a1d..878c67e393334 100644
--- a/pandas/tests/io/parser/test_network.py
+++ b/pandas/tests/io/parser/test_network.py
@@ -69,6 +69,7 @@ def tips_df(datapath):
 
 @pytest.mark.usefixtures("s3_resource")
 @td.skip_if_not_us_locale()
+@pytest.mark.single
 class TestS3:
     @td.skip_if_no("s3fs")
     def test_parse_public_s3_bucket(self, tips_df, s3so):
diff --git a/pandas/tests/io/test_fsspec.py b/pandas/tests/io/test_fsspec.py
index 666da677d702e..2c5b4f6cc874e 100644
--- a/pandas/tests/io/test_fsspec.py
+++ b/pandas/tests/io/test_fsspec.py
@@ -131,6 +131,7 @@ def test_fastparquet_options(fsspectest):
 
 
 @td.skip_if_no("s3fs")
+@pytest.mark.single
 def test_from_s3_csv(s3_resource, tips_file, s3so):
     tm.assert_equal(
         read_csv("s3://pandas-test/tips.csv", storage_options=s3so), read_csv(tips_file)
@@ -148,6 +149,7 @@ def test_from_s3_csv(s3_resource, tips_file, s3so):
 
 @pytest.mark.parametrize("protocol", ["s3", "s3a", "s3n"])
 @td.skip_if_no("s3fs")
+@pytest.mark.single
 def test_s3_protocols(s3_resource, tips_file, protocol, s3so):
     tm.assert_equal(
         read_csv("%s://pandas-test/tips.csv" % protocol, storage_options=s3so),
@@ -157,6 +159,7 @@ def test_s3_protocols(s3_resource, tips_file, protocol, s3so):
 
 @td.skip_if_no("s3fs")
 @td.skip_if_no("fastparquet")
+@pytest.mark.single
 def test_s3_parquet(s3_resource, s3so):
     fn = "s3://pandas-test/test.parquet"
     df1.to_parquet(
diff --git a/pandas/tests/io/test_parquet.py b/pandas/tests/io/test_parquet.py
index 4e0c16c71a6a8..9edf141c91eae 100644
--- a/pandas/tests/io/test_parquet.py
+++ b/pandas/tests/io/test_parquet.py
@@ -541,6 +541,7 @@ def test_categorical(self, pa):
             expected = df.astype(object)
             check_round_trip(df, pa, expected=expected)
 
+    @pytest.mark.single
     def test_s3_roundtrip_explicit_fs(self, df_compat, s3_resource, pa, s3so):
         s3fs = pytest.importorskip("s3fs")
         if LooseVersion(pyarrow.__version__) <= LooseVersion("0.17.0"):
@@ -555,6 +556,7 @@ def test_s3_roundtrip_explicit_fs(self, df_compat, s3_resource, pa, s3so):
             write_kwargs=kw,
         )
 
+    @pytest.mark.single
     def test_s3_roundtrip(self, df_compat, s3_resource, pa):
         if LooseVersion(pyarrow.__version__) <= LooseVersion("0.17.0"):
             pytest.skip()
@@ -563,6 +565,7 @@ def test_s3_roundtrip(self, df_compat, s3_resource, pa):
 
     @td.skip_if_no("s3fs")
     @pytest.mark.parametrize("partition_col", [["A"], []])
+    @pytest.mark.single
     def test_s3_roundtrip_for_dir(self, df_compat, s3_resource, pa, partition_col):
         # GH #26388
         expected_df = df_compat.copy()
@@ -761,6 +764,7 @@ def test_filter_row_groups(self, fp):
             result = read_parquet(path, fp, filters=[("a", "==", 0)])
         assert len(result) == 1
 
+    @pytest.mark.single
     def test_s3_roundtrip(self, df_compat, s3_resource, fp):
         # GH #19134
         check_round_trip(df_compat, fp, path="s3://pandas-test/fastparquet.parquet")