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rmats sashimi plots #295

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megeadsforth opened this issue May 19, 2023 · 1 comment
Open

rmats sashimi plots #295

megeadsforth opened this issue May 19, 2023 · 1 comment

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@megeadsforth
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Hi,

I have a question regarding how the sashimi plots are generated and what the numbers above the exon junctions represent.

Screenshot 2023-05-19 at 16 04 48

I don't understand how the numbers on the sashimi plot correspond to the inclusion and skipping junction counts (IJC1, IJC2 etc.) given in the output spreadsheet (shown below)

image

How are the IJC and SJC counts calculated from the raw RPKM counts?

@EricKutschera
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This post discusses the differences in counts between rMATS and rmats2sashimiplot: Xinglab/rmats2sashimiplot#33

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