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#RPGC (per bin) = number of reads per bin / scaling factor for 1x average coverage. This scaling factor, in turn, is determined from the sequencing depth: (total number of mapped reads * fragment length) / effective genome size.
#The scaling factor used is the inverse of the sequencing depth computed for the sample to match the 1x coverage. This option requires –effectiveGenomeSize.
#Each read is considered independently, if you want to only count one mate from a pair in paired-end data, then use the –samFlagInclude/–samFlagExclude options.
--effectiveGenomeSize 2652783500 #normalise to mouse genome size with multimapping - if samples are not multimapped use 2308125349 instead