diff --git a/environment.yml b/environment.yml
index 9d23b19a3..c0f3c6503 100644
--- a/environment.yml
+++ b/environment.yml
@@ -19,7 +19,7 @@ dependencies:
   # Biotite dependencies
   - msgpack-python >=0.5.6
   - networkx >=2.0
-  - numpy >=1.15
+  - numpy >=1.15, <2.0
   - requests >=2.12
   # Testing
   - mdtraj >=1.9.3
diff --git a/pyproject.toml b/pyproject.toml
index 69b15ac1f..d2f3c26c6 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -26,7 +26,7 @@ classifiers = [
 # this should be manually updated as the minimum python version increases
 dependencies = [
   "requests >= 2.12",
-  "numpy >= 1.14.5",
+  "numpy >= 1.14.5, <= 2.0",
   "msgpack >= 0.5.6",
   "networkx >= 2.0",
 ]