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RepeatAnalysisFastqToSplitFasta.py
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#! /usr/bin/env python
## Copyright (c) 2017, Pacific Biosciences of California, Inc.
##
## All rights reserved.
##
## Redistribution and use in source and binary forms, with or without
## modification, are permitted (subject to the limitations in the
## disclaimer below) provided that the following conditions are met:
##
## * Redistributions of source code must retain the above copyright
## notice, this list of conditions and the following disclaimer.
##
## * Redistributions in binary form must reproduce the above
## copyright notice, this list of conditions and the following
## disclaimer in the documentation and/or other materials provided
## with the distribution.
##
## * Neither the name of Pacific Biosciences nor the names of its
## contributors may be used to endorse or promote products derived
## from this software without specific prior written permission.
##
## NO EXPRESS OR IMPLIED LICENSES TO ANY PARTY'S PATENT RIGHTS ARE
## GRANTED BY THIS LICENSE. THIS SOFTWARE IS PROVIDED BY PACIFIC
## BIOSCIENCES AND ITS CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED
## WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES
## OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE
## DISCLAIMED. IN NO EVENT SHALL PACIFIC BIOSCIENCES OR ITS
## CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL,
## SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT
## LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF
## USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND
## ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY,
## OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT
## OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF
## SUCH DAMAGE.
## Author: Brett Bowman
import sys
from pbcore.io import FastqReader, FastaRecord, FastaWriter
outputPrefix = sys.argv[1]
fns = sys.argv[2:]
seqIds = set()
for fn in fns:
for record in FastqReader(fn):
if record.id in seqIds:
print "ERROR: Duplicate sequence id '{0}'".format(record.id)
raise SystemExit
with FastaWriter( outputPrefix + "." + record.id + ".fasta" ) as handle:
fasta = FastaRecord(record.id, record.sequence)
handle.writeRecord( fasta )
seqIds.add( record.id )