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MIRA failed during mapping assembly #31

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ghost opened this issue Jan 13, 2018 · 0 comments
Open

MIRA failed during mapping assembly #31

ghost opened this issue Jan 13, 2018 · 0 comments

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@ghost
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ghost commented Jan 13, 2018

As a test, I tried the mapping assembly in tutorial I. It succeed, which shows that there are not problems in the tools. But when I try to use my data to assmbly the chloroplast genome, there is always a failure happened.
default
Above shows the log file generated after the MITObim.pl step.
I don't know how to fix this problem. Hope your help!
Plus: I send my bash script to the server by using "qsub".
This is the script during the process:
MITObim.pl -start 1 -end 10 -sample 696 -ref ref -readpool 696_R12.fastq -maf ./696asm_assembly/696asm_d_results/696asm_out.maf --kbait 39 --platform solexa --clean yes --min_cov 20 --min_len 200 --trimreads yes --redirect_tmp /home/jimeiyuan/MIRA_tmp/ --NFS_warn_only >& log_MITObim
I set the NFS_check parameter to warn and redirected to a new directory.
Anyone knows what the problem was and how to figure it? Thank you for your help.

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