diff --git a/eng/native/configurecompiler.cmake b/eng/native/configurecompiler.cmake
index 787eea52392c14..e8ad8615f6d4bd 100644
--- a/eng/native/configurecompiler.cmake
+++ b/eng/native/configurecompiler.cmake
@@ -70,8 +70,11 @@ if (MSVC)
   add_compile_options($<$<COMPILE_LANGUAGE:CXX>:$<TARGET_PROPERTY:CLR_EH_OPTION>>)
   add_link_options($<$<BOOL:$<TARGET_PROPERTY:CLR_CONTROL_FLOW_GUARD>>:/guard:cf>)
 
-  # Load all imported DLLs from the System32 directory.
-  add_linker_flag(/DEPENDENTLOADFLAG:0x800)
+  if (NOT CLR_CMAKE_PGO_INSTRUMENT)
+    # Load all imported DLLs from the System32 directory.
+    # Don't do this when instrumenting for PGO as a local DLL dependency is introduced by the instrumentation
+    add_linker_flag(/DEPENDENTLOADFLAG:0x800)
+  endif()
 
   # Linker flags
   #