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canu polishing with CLR reads #208

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botellaflotante opened this issue Feb 11, 2022 · 1 comment
Open

canu polishing with CLR reads #208

botellaflotante opened this issue Feb 11, 2022 · 1 comment

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@botellaflotante
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Hello, I have a canu assembly with CLR reads (ca. 30X coverage). I used racon for polishing with the same reads... I got as a result much larger contigs. I don't have a reference genome to compare if what racon did I right as it seems based on contig lengths... Is this approach supposed to work well, or only illumina reads are recommended for improving this kind of assembly?

thanks

@rvaser
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rvaser commented Feb 14, 2022

Hello,
it can happen that Racon extends a given assembly. Try to evaluate with BUSCO or if you have cdna data run paftools asmgene.

Best regards,
Robert

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