Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

overlapping transcripts? --jaccard_clip with Trinity #4

Open
johnsolk opened this issue Aug 18, 2016 · 0 comments
Open

overlapping transcripts? --jaccard_clip with Trinity #4

johnsolk opened this issue Aug 18, 2016 · 0 comments

Comments

@johnsolk
Copy link
Owner

Are transcripts overlapping?
http://pasapipeline.github.io/

Note
When applying Trinity to RNA-Seq samples derived from microbial eukaryotes, using either genome-free or genome-guided de novo assembly, be sure to use the --jaccard_clip parameter to reduce the occurrence of falsely-fused genome-neighboring transcripts. Also, only include Cufflinks transcripts if applying the approach to expansive genomes of animals such as mouse or human, and exclude Cufflinks from application to compact microbial eukaryotic genomes.
Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant