index.rst

London Biohackspace SOP Index

Biolab Rules and Guidelines

BioLab Rules:	London Biohackspace BioLab Rules
BioLab Usage Guidelines:	London Biohackspace BioLab Usage Guidelines

1. Introduction

LBL01001:

Introduction to the London Biohackspace Standard Operating Procedures

2. Health and Safety Prerequisites, Inductions and Training

LBL02001:

Laboratory Induction

3. Roles and Responsibilities

LBL03001:	Roles and Responsibilities of the BSO
LBL03002:	Composition, Roles, and Responsibilities of the Safety Committee
LBL03003:	Responsibilities of Principle Investigators and Lab Users

4. General BioLab Procedures

LBL04001:	Operating the Spectrophotometer
LBL04002:	Using the Autoclave for Media Preparation
LBL04003:	The BioLab Centrifuges and their usage
LBL04004:	Using The Incubator
LBL04005:	Operating the Laminar Flow Unit for Aseptic Sample Handling
LBL04006:	Using the Autoclave for Waste Deactivation
LBL04007:	Using the Kill Bin
LBL04008:	Using the Microwave
LBL04009:	Cold storage

5. CL1 Requirements

LBL05001:

Containment Level One For BioLab Users

6. Risk Assessments and requirements for PIs

LBL06001:	Project Risk Assessment Procedure for Lab Users Proposing New Projects
LBL06002:	Risk Assessment Procedure for Transport of GMOs Outside the Lab

7. Molecular Biology Procedures

LBL07001:	Preparation of competent E. Coli (chemical method)
LBL07002:	Heat shock transformation of E. coli
LBL07003:	Gel preparation and electrophoresis
LBL07004:	Setting up and running PCRs e.g Colony PCR
LBL07006:	UV Illumination of electrophoresis gels

8. Maintenance

LBL08001:	Maintenance and Cleaning
LBL08002:	Topping up the RO water level in the Autoclave
LBL08003:	Handling spillage of micro-organisms

9. Hazardous substances

LBL09001:

Handling, Storage, and Disposal of Ethidium Bromide (EtBr)

10. Reporting Accidents and Incidents

LBL10001:

Reporting accidents and incidents