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In some cases the UMIs are provided as a third file. To get the files ready for the current version we have to add the UMIs to one of the fastQ files first and then proceed --with_umi option.
As discussed on slack#rnaseq, adding the third file breaks samplesheet format and the downstream channels/logic.
It would be simple and straightforward to handle this scenario outside the pipeline to get UMIs in both the read files and simply skip umi_tools extract but still traverse through the umi_tools dedup path.
The text was updated successfully, but these errors were encountered:
Description of feature
In some cases the UMIs are provided as a third file. To get the files ready for the current version we have to add the UMIs to one of the fastQ files first and then proceed
--with_umi
option.As discussed on slack#rnaseq, adding the third file breaks samplesheet format and the downstream channels/logic.
It would be simple and straightforward to handle this scenario outside the pipeline to get UMIs in both the read files and simply skip
umi_tools extract
but still traverse through theumi_tools dedup
path.The text was updated successfully, but these errors were encountered: