diff --git a/CHANGELOG.md b/CHANGELOG.md
index a74672fe37..3174f84e8a 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -27,6 +27,7 @@
 - Add `--fail-warned` flag to `nf-core lint` to make warnings fail ([#1593](https://github.com/nf-core/tools/pull/1593))
 - Add `--fail-warned` flag to pipeline linting workflow ([#1593](https://github.com/nf-core/tools/pull/1593))
 - Updated the package requirements to prevent defunct installations of nf-core ([#1620](https://github.com/nf-core/tools/pull/1620))
+- Remove dependency of the mock package and use unittest.mock instead ([#1696](https://github.com/nf-core/tools/pull/1696))
 - Fix and improve broken test for Singularity container download ([#1622](https://github.com/nf-core/tools/pull/1622))
 - Use [`$XDG_CACHE_HOME`](https://specifications.freedesktop.org/basedir-spec/basedir-spec-latest.html) or `~/.cache` instead of `$XDG_CONFIG_HOME` or `~/config/` as base directory for API cache
 - Switch CI to use [setup-nextflow](https://github.com/nf-core/setup-nextflow) action to install Nextflow ([#1650](https://github.com/nf-core/tools/pull/1650))
diff --git a/requirements-dev.txt b/requirements-dev.txt
index 37094ce491..6557d215d9 100644
--- a/requirements-dev.txt
+++ b/requirements-dev.txt
@@ -1,6 +1,5 @@
 pytest-datafiles
 pytest-cov
-mock
 black
 refgenie
 Sphinx
diff --git a/tests/test_cli.py b/tests/test_cli.py
index 4bfc591407..be1c300a1a 100644
--- a/tests/test_cli.py
+++ b/tests/test_cli.py
@@ -3,8 +3,8 @@
 """
 
 import unittest
+from unittest import mock
 
-import mock
 from click.testing import CliRunner
 
 import nf_core.__main__
diff --git a/tests/test_download.py b/tests/test_download.py
index c58acd6ed2..21a8e2e9a0 100644
--- a/tests/test_download.py
+++ b/tests/test_download.py
@@ -7,8 +7,8 @@
 import shutil
 import tempfile
 import unittest
+from unittest import mock
 
-import mock
 import pytest
 
 import nf_core.create
diff --git a/tests/test_launch.py b/tests/test_launch.py
index 12e4d26e5d..f3b1d469be 100644
--- a/tests/test_launch.py
+++ b/tests/test_launch.py
@@ -7,8 +7,7 @@
 import shutil
 import tempfile
 import unittest
-
-import mock
+from unittest import mock
 
 import nf_core.create
 import nf_core.launch
diff --git a/tests/test_lint.py b/tests/test_lint.py
index f75840a906..b7275c8c4b 100644
--- a/tests/test_lint.py
+++ b/tests/test_lint.py
@@ -8,8 +8,8 @@
 import subprocess
 import tempfile
 import unittest
+from unittest import mock
 
-import mock
 import pytest
 import requests
 import yaml
diff --git a/tests/test_list.py b/tests/test_list.py
index e51b8969be..f71863cbca 100644
--- a/tests/test_list.py
+++ b/tests/test_list.py
@@ -9,8 +9,8 @@
 import unittest
 from datetime import datetime
 from pathlib import Path
+from unittest import mock
 
-import mock
 import pytest
 from rich.console import Console
 
diff --git a/tests/test_schema.py b/tests/test_schema.py
index 02bf063d8e..0dd22243c4 100644
--- a/tests/test_schema.py
+++ b/tests/test_schema.py
@@ -7,9 +7,9 @@
 import shutil
 import tempfile
 import unittest
+from unittest import mock
 
 import click
-import mock
 import pytest
 import requests
 import yaml
diff --git a/tests/test_sync.py b/tests/test_sync.py
index 3d9573251b..7a6848f9cb 100644
--- a/tests/test_sync.py
+++ b/tests/test_sync.py
@@ -7,9 +7,9 @@
 import shutil
 import tempfile
 import unittest
+from unittest import mock
 
 import git
-import mock
 
 import nf_core.create
 import nf_core.sync
diff --git a/tests/test_utils.py b/tests/test_utils.py
index 12180efa0b..dd972a7375 100644
--- a/tests/test_utils.py
+++ b/tests/test_utils.py
@@ -6,8 +6,8 @@
 import shutil
 import tempfile
 import unittest
+from unittest import mock
 
-import mock
 import pytest
 import requests