############################################################ Pre-Processing ########################################################### 2 sequences in genome file TIR-Learner ############################################################ Module 1 Begin ########################################################### Module 1, Step 1: Blast Genome against Reference Library Building a new DB, current time: 06/24/2019 09:19:19 New DB name: genome.fasta-+-db New DB title: genome.fasta Sequence type: Nucleotide Keep MBits: T Maximum file size: 1000000000B Adding sequences from FASTA; added 2 sequences in 0.040338 seconds. FASTA-Reader: Ignoring invalid residues at position(s): On line 250: 1224-1597, 1622-1750 FASTA-Reader: Ignoring invalid residues at position(s): On line 252: 520-746 FASTA-Reader: Ignoring invalid residues at position(s): On line 254: 1936-2299 FASTA-Reader: Ignoring invalid residues at position(s): On line 380: 5993-6353 FASTA-Reader: Ignoring invalid residues at position(s): On line 386: 238-242, 1481-1485, 2106-2110, 3127-3131 FASTA-Reader: Ignoring invalid residues at position(s): On line 472: 268-272 FASTA-Reader: Ignoring invalid residues at position(s): On line 534: 225-229 FASTA-Reader: Ignoring invalid residues at position(s): On line 566: 11-15 No Blast Result, Jump to Module3 ############################################################ Module 3 Begin ########################################################### Module 3, Step 1: Split Genome and Run GRF program to find Inverted Repeats start: Mon Jun 24 09:19:33 2019 calculate cumulative scores in chromosome scaffold_2: Mon Jun 24 09:19:33 2019 find candidates in chromosome scaffold_2: Mon Jun 24 09:19:33 2019 print: Mon Jun 24 09:23:59 2019 end: Mon Jun 24 09:23:59 2019 start: Mon Jun 24 09:23:59 2019 calculate cumulative scores in chromosome scaffold_1: Mon Jun 24 09:23:59 2019 find candidates in chromosome scaffold_1: Mon Jun 24 09:23:59 2019 print: Mon Jun 24 09:29:28 2019 end: Mon Jun 24 09:29:29 2019 Module 2, Step 2: Process GRF results rm: cannot remove 'TIR-Learner-+-scaffold_1.fa': No such file or directory rm: cannot remove 'TIR-Learner-+-scaffold_2.fa': No such file or directory Module 3, Step 3: Get dataset Module 3, Step 4: ML prediction Module 3, Step 5: Check TIR/TSD rm: cannot remove '*_DTA.fa': No such file or directory rm: cannot remove '*_DTC.fa': No such file or directory rm: cannot remove '*_DTH.fa': No such file or directory rm: cannot remove '*_DTM.fa': No such file or directory rm: cannot remove '*_DTT.fa': No such file or directory Module 3, Step 6: Write to Gff Module 3, Step 7: Remove Low complexity ############################################################ Module 3 Finished ########################################################### Get Final GFF cp: cannot stat 'Module3/TIR-Learner/TIR-Learner-+-Module3.gff3': No such file or directory cp: cannot stat 'Module3/TIR-Learner/TIR-Learner-+-Module3-+-Low': No such file or directory ############################################################################## Removing Entries with Homology in Flanking Sequences ################################## Traceback (most recent call last): File "~/bin/EDTA-TIR-Learner1.13/bin/TIR-Learner1.13/Module3_New/CombineAll.py", line 91, in remove = FlankingBlast("%sLow"%(genome_Name+spliter+dataset+spliter)) File "~/bin/EDTA-TIR-Learner1.13/bin/TIR-Learner1.13/Module3_New/CombineAll.py", line 37, in FlankingBlast f=open(file,"r+") FileNotFoundError: [Errno 2] No such file or directory: 'TIR-Learner-+-Module3-+-Low' mv: cannot stat '*.gff3': No such file or directory rm: cannot remove '*Low': No such file or directory Get fasta file Traceback (most recent call last): File "~/bin/EDTA-TIR-Learner1.13/bin/TIR-Learner1.13/Module3_New/GetAllSeq.py", line 60, in file=open(f,"r+") FileNotFoundError: [Errno 2] No such file or directory: 'TIR-Learner_FinalAnn.gff3' mv: cannot stat 'TIR-Learner/*FinalAnn.gff3': No such file or directory mv: cannot stat 'TIR-Learner/*FinalAnn.fa': No such file or directory ############################################################ TIR-Learner is finished! ###########################################################