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I'm using the fastq-mcf (1.04.662) in my pipeline to filter adaptors and trim
reads by quality.
I have used it for a methylation sequencing run and I didn't change the skew
parameter, I left the default parameter -k=2.
I would like to know if would be better to re-run with k=0, just like in the
help, what is the impact in the reads with -k=2 ?
I would like to know what the -k parameter does to the sequences, it will trim
them ?
I look at some fastq-mcf reports and show the below messages for the positions
from 1 to 49:
Within-read Skew: Position XXX from the start of reads is skewed!
Within-read Skew: Position XXX from the end of reads is skewed!
what this means ? This positions were trimmed ?
Best regards,
Milton
Original issue reported on code.google.com by [email protected] on 7 May 2015 at 2:53
The text was updated successfully, but these errors were encountered:
Original issue reported on code.google.com by
[email protected]
on 7 May 2015 at 2:53The text was updated successfully, but these errors were encountered: