Starting Trycycler MSA (2020-07-07 09:52:59) Trycycler MSA is a tool for conducting global multiple sequence alignment of contig sequences. Input sequences: A_contig_1: 1,038,291 bp B_contig_2: 1,038,269 bp C_contig_1: 1,038,379 bp D_contig_1: 1,038,384 bp E_contig_1: 1,038,433 bp G_utg000001c: 1,042,913 bp H_utg000001c: 1,042,821 bp I_utg000001c: 1,042,871 bp J_utg000001c: 1,042,500 bp Checking required software: MUSCLE: v3.8.1551 Partitioning sequences (2020-07-07 09:52:59) The sequences are now partitioned into smaller chunks to make the multiple sequence alignment more tractable. pieces: 719 median piece size: 1,000 bp max piece size: 7,000 bp Running Muscle (2020-07-07 09:53:12) Trycycler now runs Muscle on each of the pieces to turn them into multiple sequence alignments. pieces: 719 Merging MSA (2020-07-07 09:56:45) Each of the MSA pieces are now merged together and saved to file. MSA length: 1,046,326 bp Saving to: trycycler/cluster_001/3_msa.fasta