Starting Trycycler MSA (2020-07-06 14:18:27) Trycycler MSA is a tool for conducting global multiple sequence alignment of contig sequences. Input sequences: A_contig_1: 1,044,291 bp B_contig_1: 1,044,295 bp C_contig_1: 1,044,313 bp D_contig_1: 1,044,336 bp E_contig_1: 1,044,318 bp F_utg000001c: 1,044,419 bp G_utg000001c: 1,044,418 bp H_utg000001c: 1,044,435 bp I_utg000001c: 1,044,409 bp J_utg000001c: 1,044,416 bp Checking required software: MUSCLE: v3.8.1551 Partitioning sequences (2020-07-06 14:18:27) The sequences are now partitioned into smaller chunks to make the multiple sequence alignment more tractable. pieces: 1025 median piece size: 1,000 bp max piece size: 3,000 bp Running Muscle (2020-07-06 14:18:41) Trycycler now runs Muscle on each of the pieces to turn them into multiple sequence alignments. pieces: 1025 Merging MSA (2020-07-06 14:19:54) Each of the MSA pieces are now merged together and saved to file. MSA length: 1,044,540 bp Saving to: trycycler/cluster_001/3_msa.fasta