diff --git a/src/Temporalio.Extensions.Hosting/ITemporalWorkerServiceOptionsBuilder.cs b/src/Temporalio.Extensions.Hosting/ITemporalWorkerServiceOptionsBuilder.cs
index 512e8417..15e43cbf 100644
--- a/src/Temporalio.Extensions.Hosting/ITemporalWorkerServiceOptionsBuilder.cs
+++ b/src/Temporalio.Extensions.Hosting/ITemporalWorkerServiceOptionsBuilder.cs
@@ -14,6 +14,11 @@ public interface ITemporalWorkerServiceOptionsBuilder
         /// </summary>
         string TaskQueue { get; }
 
+        /// <summary>
+        /// Gets the build ID for this worker service. If unset, versioning is disabled.
+        /// </summary>
+        string? BuildId { get; }
+
         /// <summary>
         /// Gets the service collection being configured.
         /// </summary>
diff --git a/src/Temporalio.Extensions.Hosting/TemporalHostingServiceCollectionExtensions.cs b/src/Temporalio.Extensions.Hosting/TemporalHostingServiceCollectionExtensions.cs
index e064ba7b..91515d84 100644
--- a/src/Temporalio.Extensions.Hosting/TemporalHostingServiceCollectionExtensions.cs
+++ b/src/Temporalio.Extensions.Hosting/TemporalHostingServiceCollectionExtensions.cs
@@ -17,9 +17,9 @@ public static class TemporalHostingServiceCollectionExtensions
         /// Add a hosted Temporal worker service as a <see cref="IHostedService" /> that contains
         /// its own client that connects with the given target and namespace. To use an injected
         /// <see cref="ITemporalClient" />, use
-        /// <see cref="AddHostedTemporalWorker(IServiceCollection, string)" />. The worker service
-        /// will be registered as a singleton. The result is an options builder that can be used to
-        /// configure the service.
+        /// <see cref="AddHostedTemporalWorker(IServiceCollection, string, string?)" />. The worker
+        /// service will be registered as a singleton. The result is an options builder that can be
+        /// used to configure the service.
         /// </summary>
         /// <param name="services">Service collection to create hosted worker on.</param>
         /// <param name="clientTargetHost">Client target host to connect to when starting the
@@ -27,37 +27,59 @@ public static class TemporalHostingServiceCollectionExtensions
         /// <param name="clientNamespace">Client namespace to connect to when starting the worker.
         /// </param>
         /// <param name="taskQueue">Task queue for the worker.</param>
+        /// <param name="buildId">
+        /// Build ID for the worker. Set to non-null to opt in to versioning. If versioning is
+        /// wanted, this must be set here and not later via configure options. This is because the
+        /// combination of task queue and build ID make up the unique identifier for a worker in the
+        /// service collection.
+        /// </param>
         /// <returns>Options builder to configure the service.</returns>
         public static ITemporalWorkerServiceOptionsBuilder AddHostedTemporalWorker(
             this IServiceCollection services,
             string clientTargetHost,
             string clientNamespace,
-            string taskQueue) =>
-            services.AddHostedTemporalWorker(taskQueue).ConfigureOptions(options =>
+            string taskQueue,
+            string? buildId = null) =>
+            services.AddHostedTemporalWorker(taskQueue, buildId).ConfigureOptions(options =>
                 options.ClientOptions = new(clientTargetHost) { Namespace = clientNamespace });
 
         /// <summary>
         /// Add a hosted Temporal worker service as a <see cref="IHostedService" /> that expects
         /// an injected <see cref="ITemporalClient" /> (or the returned builder
         /// can have client options populated). Use
-        /// <see cref="AddHostedTemporalWorker(IServiceCollection, string, string, string)" /> to
-        /// not expect an injected instance and instead connect to a client on worker start. The
+        /// <see cref="AddHostedTemporalWorker(IServiceCollection, string, string, string, string?)" />
+        /// to not expect an injected instance and instead connect to a client on worker start. The
         /// worker service will be registered as a singleton. The result is an options builder that
         /// can be used to configure the service.
         /// </summary>
         /// <param name="services">Service collection to create hosted worker on.</param>
         /// <param name="taskQueue">Task queue for the worker.</param>
+        /// <param name="buildId">
+        /// Build ID for the worker. Set to non-null to opt in to versioning. If versioning is
+        /// wanted, this must be set here and not later via configure options. This is because the
+        /// combination of task queue and build ID make up the unique identifier for a worker in the
+        /// service collection.
+        /// </param>
         /// <returns>Options builder to configure the service.</returns>
         public static ITemporalWorkerServiceOptionsBuilder AddHostedTemporalWorker(
-            this IServiceCollection services, string taskQueue)
+            this IServiceCollection services, string taskQueue, string? buildId = null)
         {
             // We have to use AddSingleton instead of AddHostedService because the latter does
             // not allow us to register multiple of the same type, see
-            // https://github.com/dotnet/runtime/issues/38751
+            // https://github.com/dotnet/runtime/issues/38751.
             services.AddSingleton<IHostedService>(provider =>
-                ActivatorUtilities.CreateInstance<TemporalWorkerService>(provider, taskQueue));
-            return new TemporalWorkerServiceOptionsBuilder(taskQueue, services).ConfigureOptions(
-                options => options.TaskQueue = taskQueue);
+                ActivatorUtilities.CreateInstance<TemporalWorkerService>(
+                    provider, (TaskQueue: taskQueue, BuildId: buildId)));
+            return new TemporalWorkerServiceOptionsBuilder(taskQueue, buildId, services).ConfigureOptions(
+                options =>
+                {
+                    options.TaskQueue = taskQueue;
+                    options.BuildId = buildId;
+                    options.UseWorkerVersioning = buildId != null;
+                },
+                // Disallow duplicate options registrations because that means multiple worker
+                // services with the same task queue + build ID were added.
+                disallowDuplicates: true);
         }
 
         /// <summary>
diff --git a/src/Temporalio.Extensions.Hosting/TemporalWorkerService.cs b/src/Temporalio.Extensions.Hosting/TemporalWorkerService.cs
index 82720398..d9534e81 100644
--- a/src/Temporalio.Extensions.Hosting/TemporalWorkerService.cs
+++ b/src/Temporalio.Extensions.Hosting/TemporalWorkerService.cs
@@ -68,11 +68,11 @@ public TemporalWorkerService(
 
         /// <summary>
         /// Initializes a new instance of the <see cref="TemporalWorkerService"/> class using
-        /// options and possibly an existing client. This constructor is mostly used by DI
-        /// containers. The task queue is used as the name on the options monitor to lookup the
-        /// options for the worker service.
+        /// options and possibly an existing client. This constructor was used by DI
+        /// containers and is now DEPRECATED.
         /// </summary>
-        /// <param name="taskQueue">Task queue which is the options name.</param>
+        /// <param name="taskQueue">Task queue which is included in the options name.</param>
+        /// <param name="buildId">Build ID which is included in the options name.</param>
         /// <param name="optionsMonitor">Used to lookup the options to build the worker with.
         /// </param>
         /// <param name="existingClient">Existing client to use if the options don't specify
@@ -80,14 +80,63 @@ public TemporalWorkerService(
         /// <param name="loggerFactory">Logger factory to use if not already on the worker options.
         /// The worker options logger factory or this one will be also be used for the client if an
         /// existing client does not exist (regardless of client options' logger factory).</param>
-        [ActivatorUtilitiesConstructor]
+        [Obsolete("Deprecated older form of DI constructor, task queue + build ID tuple one is used instead.")]
         public TemporalWorkerService(
             string taskQueue,
+            string? buildId,
             IOptionsMonitor<TemporalWorkerServiceOptions> optionsMonitor,
             ITemporalClient? existingClient = null,
             ILoggerFactory? loggerFactory = null)
+            : this((taskQueue, buildId), optionsMonitor, existingClient, loggerFactory)
         {
-            var options = (TemporalWorkerServiceOptions)optionsMonitor.Get(taskQueue).Clone();
+        }
+
+        /// <summary>
+        /// Initializes a new instance of the <see cref="TemporalWorkerService"/> class using
+        /// options and possibly an existing client. This constructor is only for use by DI
+        /// containers. The task queue and build ID are used as the name for the options monitor to
+        /// lookup the options for the worker service.
+        /// </summary>
+        /// <param name="taskQueueAndBuildId">Task queue and build ID for the options name.</param>
+        /// <param name="optionsMonitor">Used to lookup the options to build the worker with.
+        /// </param>
+        /// <param name="existingClient">Existing client to use if the options don't specify
+        /// client connection options (connected when run if lazy and not connected).</param>
+        /// <param name="loggerFactory">Logger factory to use if not already on the worker options.
+        /// The worker options logger factory or this one will be also be used for the client if an
+        /// existing client does not exist (regardless of client options' logger factory).</param>
+        /// <remarks>
+        /// WARNING: Do not rely on the signature of this constructor, it is for DI container use
+        /// only and may change in incompatible ways.
+        /// </remarks>
+        [ActivatorUtilitiesConstructor]
+        public TemporalWorkerService(
+            (string TaskQueue, string? BuildId) taskQueueAndBuildId,
+            IOptionsMonitor<TemporalWorkerServiceOptions> optionsMonitor,
+            ITemporalClient? existingClient = null,
+            ILoggerFactory? loggerFactory = null)
+        {
+            var options = (TemporalWorkerServiceOptions)optionsMonitor.Get(
+                TemporalWorkerServiceOptions.GetUniqueOptionsName(
+                    taskQueueAndBuildId.TaskQueue, taskQueueAndBuildId.BuildId)).Clone();
+
+            // Make sure options values match the ones given in constructor
+            if (options.TaskQueue != taskQueueAndBuildId.TaskQueue)
+            {
+                throw new InvalidOperationException(
+                    $"Task queue '{taskQueueAndBuildId.TaskQueue}' on constructor different than '{options.TaskQueue}' on options");
+            }
+            if (options.BuildId != taskQueueAndBuildId.BuildId)
+            {
+                throw new InvalidOperationException(
+                    $"Build ID '{taskQueueAndBuildId.BuildId ?? "<unset>"}' on constructor different than '{options.BuildId ?? "<unset>"}' on options");
+            }
+            if (options.UseWorkerVersioning != (taskQueueAndBuildId.BuildId != null))
+            {
+                throw new InvalidOperationException(
+                    $"Use versioning option is {options.UseWorkerVersioning}, but constructor expects different");
+            }
+
             newClientOptions = options.ClientOptions;
             if (newClientOptions == null)
             {
diff --git a/src/Temporalio.Extensions.Hosting/TemporalWorkerServiceOptions.cs b/src/Temporalio.Extensions.Hosting/TemporalWorkerServiceOptions.cs
index 1e95e8f8..21c0466f 100644
--- a/src/Temporalio.Extensions.Hosting/TemporalWorkerServiceOptions.cs
+++ b/src/Temporalio.Extensions.Hosting/TemporalWorkerServiceOptions.cs
@@ -41,5 +41,20 @@ public override object Clone()
             }
             return options;
         }
+
+        /// <summary>
+        /// Get an options name for the given task queue and build ID.
+        /// </summary>
+        /// <param name="taskQueue">Task queue.</param>
+        /// <param name="buildId">Build ID.</param>
+        /// <returns>Unique string name for the options.</returns>
+        internal static string GetUniqueOptionsName(string taskQueue, string? buildId)
+        {
+            if (buildId == null)
+            {
+                return taskQueue;
+            }
+            return taskQueue + "!!__temporal__!!" + buildId;
+        }
     }
 }
\ No newline at end of file
diff --git a/src/Temporalio.Extensions.Hosting/TemporalWorkerServiceOptionsBuilder.cs b/src/Temporalio.Extensions.Hosting/TemporalWorkerServiceOptionsBuilder.cs
index c125f51e..b3f3d5ea 100644
--- a/src/Temporalio.Extensions.Hosting/TemporalWorkerServiceOptionsBuilder.cs
+++ b/src/Temporalio.Extensions.Hosting/TemporalWorkerServiceOptionsBuilder.cs
@@ -14,14 +14,30 @@ public class TemporalWorkerServiceOptionsBuilder : ITemporalWorkerServiceOptions
         /// <param name="taskQueue">Task queue for the worker.</param>
         /// <param name="services">Service collection being configured.</param>
         public TemporalWorkerServiceOptionsBuilder(string taskQueue, IServiceCollection services)
+            : this(taskQueue, null, services)
+        {
+        }
+
+        /// <summary>
+        /// Initializes a new instance of the <see cref="TemporalWorkerServiceOptionsBuilder" />
+        /// class.
+        /// </summary>
+        /// <param name="taskQueue">Task queue for the worker.</param>
+        /// <param name="buildId">Build ID for the worker.</param>
+        /// <param name="services">Service collection being configured.</param>
+        public TemporalWorkerServiceOptionsBuilder(string taskQueue, string? buildId, IServiceCollection services)
         {
             TaskQueue = taskQueue;
+            BuildId = buildId;
             Services = services;
         }
 
         /// <inheritdoc />
         public string TaskQueue { get; private init; }
 
+        /// <inheritdoc />
+        public string? BuildId { get; private init; }
+
         /// <inheritdoc />
         public IServiceCollection Services { get; private init; }
     }
diff --git a/src/Temporalio.Extensions.Hosting/TemporalWorkerServiceOptionsBuilderExtensions.cs b/src/Temporalio.Extensions.Hosting/TemporalWorkerServiceOptionsBuilderExtensions.cs
index de6d8aef..a29fca86 100644
--- a/src/Temporalio.Extensions.Hosting/TemporalWorkerServiceOptionsBuilderExtensions.cs
+++ b/src/Temporalio.Extensions.Hosting/TemporalWorkerServiceOptionsBuilderExtensions.cs
@@ -1,4 +1,5 @@
 using System;
+using System.Linq;
 using Microsoft.Extensions.DependencyInjection;
 using Microsoft.Extensions.DependencyInjection.Extensions;
 using Microsoft.Extensions.Options;
@@ -167,22 +168,46 @@ public static ITemporalWorkerServiceOptionsBuilder AddWorkflow(
         /// Get an options builder to configure worker service options.
         /// </summary>
         /// <param name="builder">Builder to use.</param>
+        /// <param name="disallowDuplicates">If true, will fail if options already registered for
+        /// this builder's task queue and build ID.</param>
         /// <returns>Options builder.</returns>
         public static OptionsBuilder<TemporalWorkerServiceOptions> ConfigureOptions(
-            this ITemporalWorkerServiceOptionsBuilder builder) =>
-            builder.Services.AddOptions<TemporalWorkerServiceOptions>(builder.TaskQueue);
+            this ITemporalWorkerServiceOptionsBuilder builder,
+            bool disallowDuplicates = false)
+        {
+            // To ensure the user isn't accidentally registering a duplicate task queue + build ID
+            // worker, we check here that there aren't duplicate options
+            var optionsName = TemporalWorkerServiceOptions.GetUniqueOptionsName(
+                    builder.TaskQueue, builder.BuildId);
+            if (disallowDuplicates)
+            {
+                var any = builder.Services.Any(s =>
+                    s.ImplementationInstance is ConfigureNamedOptions<TemporalWorkerServiceOptions> instance &&
+                    instance.Name == optionsName);
+                if (any)
+                {
+                    throw new InvalidOperationException(
+                        $"Worker service for task queue '{builder.TaskQueue}' and build ID '{builder.BuildId ?? "<unset>"}' already on collection");
+                }
+            }
+
+            return builder.Services.AddOptions<TemporalWorkerServiceOptions>(optionsName);
+        }
 
         /// <summary>
         /// Configure worker service options using an action.
         /// </summary>
         /// <param name="builder">Builder to use.</param>
         /// <param name="configure">Configuration action.</param>
+        /// <param name="disallowDuplicates">If true, will fail if options already registered for
+        /// this builder's task queue and build ID.</param>
         /// <returns>Same builder instance.</returns>
         public static ITemporalWorkerServiceOptionsBuilder ConfigureOptions(
             this ITemporalWorkerServiceOptionsBuilder builder,
-            Action<TemporalWorkerServiceOptions> configure)
+            Action<TemporalWorkerServiceOptions> configure,
+            bool disallowDuplicates = false)
         {
-            builder.ConfigureOptions().Configure(configure);
+            builder.ConfigureOptions(disallowDuplicates).Configure(configure);
             return builder;
         }
 
diff --git a/tests/Temporalio.Tests/Extensions/Hosting/TemporalWorkerServiceTests.cs b/tests/Temporalio.Tests/Extensions/Hosting/TemporalWorkerServiceTests.cs
index fb82db6b..ae3494d3 100644
--- a/tests/Temporalio.Tests/Extensions/Hosting/TemporalWorkerServiceTests.cs
+++ b/tests/Temporalio.Tests/Extensions/Hosting/TemporalWorkerServiceTests.cs
@@ -202,4 +202,72 @@ public async Task TemporalWorkerService_ExecuteAsync_MultipleWorkers()
             },
             result);
     }
+
+    [Workflow("Workflow")]
+    public class WorkflowV1
+    {
+        [WorkflowRun]
+        public async Task<string> RunAsync() => "done-v1";
+    }
+
+    [Workflow("Workflow")]
+    public class WorkflowV2
+    {
+        [WorkflowRun]
+        public async Task<string> RunAsync() => "done-v2";
+    }
+
+    [Fact]
+    public async Task TemporalWorkerService_ExecuteAsync_MultipleVersionsSameQueue()
+    {
+        var taskQueue = $"tq-{Guid.NewGuid()}";
+        // Build with two workers on same queue but different versions
+        var bld = Host.CreateApplicationBuilder();
+        bld.Services.AddSingleton(Client);
+        bld.Services.
+            AddHostedTemporalWorker(taskQueue, "1.0").
+            AddWorkflow<WorkflowV1>();
+        bld.Services.
+            AddHostedTemporalWorker(taskQueue, "2.0").
+            AddWorkflow<WorkflowV2>();
+
+        // Start the host
+        using var tokenSource = new CancellationTokenSource();
+        using var host = bld.Build();
+        var hostTask = Task.Run(() => host.RunAsync(tokenSource.Token));
+
+        // Set 1.0 as default and run
+        await Env.Client.UpdateWorkerBuildIdCompatibilityAsync(
+            taskQueue, new BuildIdOp.AddNewDefault("1.0"));
+        var res = await Client.ExecuteWorkflowAsync(
+            (WorkflowV1 wf) => wf.RunAsync(),
+            new($"wf-{Guid.NewGuid()}", taskQueue));
+        Assert.Equal("done-v1", res);
+
+        // Update default and run again
+        await Env.Client.UpdateWorkerBuildIdCompatibilityAsync(
+            taskQueue, new BuildIdOp.AddNewDefault("2.0"));
+        res = await Client.ExecuteWorkflowAsync(
+            (WorkflowV1 wf) => wf.RunAsync(),
+            new($"wf-{Guid.NewGuid()}", taskQueue));
+        Assert.Equal("done-v2", res);
+    }
+
+    [Fact]
+    public async Task TemporalWorkerService_ExecuteAsync_DuplicateQueue()
+    {
+        var taskQueue = $"tq-{Guid.NewGuid()}";
+        // Build with two workers on same queue but different versions
+        var bld = Host.CreateApplicationBuilder();
+        bld.Services.AddSingleton(Client);
+        bld.Services.
+            AddHostedTemporalWorker(taskQueue).
+            AddWorkflow<WorkflowV1>();
+        var exc = Assert.Throws<InvalidOperationException>(() =>
+            bld.Services.
+                AddHostedTemporalWorker(taskQueue).
+                AddWorkflow<WorkflowV2>());
+        Assert.StartsWith("Worker service", exc.Message);
+        Assert.EndsWith("already on collection", exc.Message);
+    }
 }
\ No newline at end of file