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run_tcoffee.pl
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#!/usr/bin/perl
###############################################################################
#
# run_tcoffee.pl
#
# Run Multiple sequence alignment using T_Coffee in batch mode.
#
# Copyright (C) 2013 Zhuofei Xu
#
# This program is free software: you can redistribute it and/or modify
# it under the terms of the GNU General Public License as published by
# the Free Software Foundation, either version 3 of the License, or
# (at your option) any later version.
#
# This program is distributed in the hope that it will be useful,
# but WITHOUT ANY WARRANTY; without even the implied warranty of
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
# GNU General Public License for more details.
#
# You should have received a copy of the GNU General Public License
# along with this program. If not, see <http://www.gnu.org/licenses/>.
#
###############################################################################
use strict;
use warnings;
#core Perl modules
use Getopt::Long;
#locally-written modules
BEGIN {
select(STDERR);
$| = 1;
select(STDOUT);
$| = 1;
}
# get input params
my $global_options = checkParams();
my $inputdir;
$inputdir = &overrideDefault("inputfile.dir",'inputdir');
######################################################################
# CODE
######################################################################
opendir(DIR, $inputdir) || die "Can't open directory\n";
my @array = ();
@array = readdir(DIR);
foreach my $file (@array){
next unless ($file =~ /^\S+.fa$/);
system ("t_coffee $inputdir/$file");
}
closedir (DIR);
######################################################################
# TEMPLATE SUBS
######################################################################
sub checkParams {
#-----
# Do any and all options checking here...
#
my @standard_options = ( "help|h+", "inputdir|i:s");
my %options;
# Add any other command line options, and the code to handle them
#
GetOptions( \%options, @standard_options );
#if no arguments supplied print the usage and exit
#
exec("pod2usage $0") if (0 == (keys (%options) ));
# If the -help option is set, print the usage and exit
#
exec("pod2usage $0") if $options{'help'};
# Compulsosy items
#if(!exists $options{'infile'} ) { print "**ERROR: $0 : \n"; exec("pod2usage $0"); }
return \%options;
}
sub overrideDefault
{
#-----
# Set and override default values for parameters
#
my ($default_value, $option_name) = @_;
if(exists $global_options->{$option_name})
{
return $global_options->{$option_name};
}
return $default_value;
}
__DATA__
=head1 NAME
run_tcoffee.pl
=head1 COPYRIGHT
Copyright (C) 2013 Zhuofei Xu
This program is free software: you can redistribute it and/or modify
it under the terms of the GNU General Public License as published by
the Free Software Foundation, either version 3 of the License, or
(at your option) any later version.
This program is distributed in the hope that it will be useful,
but WITHOUT ANY WARRANTY; without even the implied warranty of
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
GNU General Public License for more details.
You should have received a copy of the GNU General Public License
along with this program. If not, see <http://www.gnu.org/licenses/>.
=head1 DESCRIPTION
Run Multiple sequence alignment using T_Coffee in batch mode.
=head1 SYNOPSIS
script.pl -i [-h]
[-help -h] Displays this basic usage information
[-inputdir -i] Input directory containing sequence files in fasta format
=cut