diff --git a/e2e/cases/web-worker/new-worker/index.test.ts b/e2e/cases/web-worker/new-worker/index.test.ts
new file mode 100644
index 0000000000..307d57533c
--- /dev/null
+++ b/e2e/cases/web-worker/new-worker/index.test.ts
@@ -0,0 +1,15 @@
+import path from 'node:path';
+import { expect, test } from '@playwright/test';
+import { build, gotoPage } from '@e2e/helper';
+
+test('should allow to build web-worker with new Worker', async ({ page }) => {
+  const rsbuild = await build({
+    cwd: __dirname,
+    runServer: true,
+  });
+
+  await gotoPage(page, rsbuild);
+  await expect(page.locator('#root')).toHaveText(
+    'The Answer to the Ultimate Question of Life, The Universe, and Everything: 42',
+  );
+});
diff --git a/e2e/cases/web-worker/new-worker/src/index.js b/e2e/cases/web-worker/new-worker/src/index.js
new file mode 100644
index 0000000000..5e6d12fa5f
--- /dev/null
+++ b/e2e/cases/web-worker/new-worker/src/index.js
@@ -0,0 +1,10 @@
+const worker = new Worker(new URL('./worker.js', import.meta.url));
+
+worker.postMessage({
+  question:
+    'The Answer to the Ultimate Question of Life, The Universe, and Everything',
+});
+
+worker.onmessage = ({ data: { answer } }) => {
+  document.getElementById('root').innerHTML = answer;
+};
diff --git a/e2e/cases/web-worker/new-worker/src/worker.js b/e2e/cases/web-worker/new-worker/src/worker.js
new file mode 100644
index 0000000000..202c294f4d
--- /dev/null
+++ b/e2e/cases/web-worker/new-worker/src/worker.js
@@ -0,0 +1,5 @@
+self.onmessage = ({ data: { question } }) => {
+  self.postMessage({
+    answer: `${question}: 42`,
+  });
+};
diff --git a/e2e/cases/web-worker/.browserslistrc b/e2e/cases/web-worker/web-worker-target/.browserslistrc
similarity index 100%
rename from e2e/cases/web-worker/.browserslistrc
rename to e2e/cases/web-worker/web-worker-target/.browserslistrc
diff --git a/e2e/cases/web-worker/index.test.ts b/e2e/cases/web-worker/web-worker-target/index.test.ts
similarity index 100%
rename from e2e/cases/web-worker/index.test.ts
rename to e2e/cases/web-worker/web-worker-target/index.test.ts
diff --git a/e2e/cases/web-worker/src/index.js b/e2e/cases/web-worker/web-worker-target/src/index.js
similarity index 100%
rename from e2e/cases/web-worker/src/index.js
rename to e2e/cases/web-worker/web-worker-target/src/index.js
diff --git a/e2e/cases/web-worker/src/index2.js b/e2e/cases/web-worker/web-worker-target/src/index2.js
similarity index 100%
rename from e2e/cases/web-worker/src/index2.js
rename to e2e/cases/web-worker/web-worker-target/src/index2.js
diff --git a/e2e/cases/web-worker/src/test.js b/e2e/cases/web-worker/web-worker-target/src/test.js
similarity index 100%
rename from e2e/cases/web-worker/src/test.js
rename to e2e/cases/web-worker/web-worker-target/src/test.js