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Create mappability file as shown in 4.4 #24

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Juergent79 opened this issue Apr 16, 2024 · 1 comment
Open

Create mappability file as shown in 4.4 #24

Juergent79 opened this issue Apr 16, 2024 · 1 comment

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@Juergent79
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Unfortunatly, I cannot reproduce the creation of the mappability file.
Using gem-mappability, I get a .wig file with variable step size
variableStep chrom=CP011454.1 Gemmatimonas phototrophica strain AP64 span=2986
1 1
variableStep chrom=CP011454.1 Gemmatimonas phototrophica strain AP64
2987 0.5
variableStep chrom=CP011454.1 Gemmatimonas phototrophica strain AP64 span=23696
2988 1
variableStep chrom=CP011454.1 Gemmatimonas phototrophica strain AP64 span=20
26684 0.5

Could you provide the exact steps to reproduce your example?

@yezhengSTAT
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yezhengSTAT commented Apr 16, 2024

I used to use the guide in https://wiki.bits.vib.be/index.php/Create_a_mappability_track to create the mappability file but I haven't checked this tool for a while. There are other researchers who may have generated the mappability files that you can use directly. For example, data and scripts at https://zenodo.org/records/5521424 seem to be useful.

Thanks,
Ye

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