cDNA sequencing

[sagnikbanerjee15]

Hello,

Can the same pipeline be used for cDNA sequencing as well? I have the fastq reads and the reference genome and transcriptome fasta sequences.

Thank you.

[yjx1217]

Hi, module 04 (RNA modification) and module 05 (polyA tail length) requires direct-RNA sequencing reads in fast5 format, whereas other modules should still work for cDNA sequencing fastq reads in theory (although I have not tested).

Best,
Jia-Xing