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# DOG | ||
A Jython script for [Fiji/ImageJ](https://github.com/fiji) to perform [Difference of Gaussians](http://en.wikipedia.org/wiki/Difference_of_Gaussians) on fluorescent stack and plot the result. | ||
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This script will open a single channel fluorescent stack, perform the Difference of Gaussians and then plot all found points with the orthoslice view | ||
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## Parameters available : | ||
- cellDiameter : select the cell/nucleus diameter (in unit of the Image Stack) | ||
- minPeak : min intensity for a peak to be considered | ||
- plotType : plot Points, Icospheres or limit to the first 3495 points due to some slowdown with Icospheres | ||
- cellIcosophere : size of the point/icosphere use to visualize each detected point. | ||
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## TODO: | ||
- Apply 3D Watershed segmentation using the detected points has a seed (see [3D Watershed plugin](http://imagejdocu.tudor.lu/doku.php?id=plugin:segmentation:3d_spots_segmentation:start#d_watershed) ) | ||
- Allow multiple channels in the stack | ||
- Add a dialog for the parameters | ||
- Add interface to measure nucleus/cell parameters |