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[![MR.PARETO](https://img.shields.io/badge/MR.PARETO-red)](https://github.com/epigen/mr.pareto/)
[![MrBiomics](https://img.shields.io/badge/MrBiomics-red)](https://github.com/epigen/MrBiomics/)
[![DOI](https://zenodo.org/badge/350342694.svg)](https://zenodo.org/doi/10.5281/zenodo.6323634)
[![](https://tokei.rs/b1/github/epigen/atacseq_pipeline?category=code)]()
[![](https://tokei.rs/b1/github/epigen/atacseq_pipeline?category=files)]()
[![GitHub license](https://img.shields.io/github/license/epigen/atacseq_pipeline)](https://github.com/epigen/atacseq_pipeline/blob/master/LICENSE)
![GitHub Release](https://img.shields.io/github/v/release/epigen/atacseq_pipeline)
[![Snakemake](https://img.shields.io/badge/Snakemake->=8.20.1-green)](https://snakemake.readthedocs.io/en/stable/)

# Ultimate ATAC-seq Data Processing & Quantification Pipeline
# Ultimate ATAC-seq Data Processing, Quantification & Annotation Pipeline
A [Snakemake 8](https://snakemake.readthedocs.io/en/stable/) workflow implementation of the [BSF's](https://www.biomedical-sequencing.org/) [ATAC-seq Data Processing Pipeline](https://github.com/berguner/atacseq_pipeline "ATAC-seq Data Processing Pipeline") extended by downstream quantification and annotation steps using Bash and Python.

> [!NOTE]
> This workflow adheres to the module specifications of [MR.PARETO](https://github.com/epigen/mr.pareto), an effort to augment research by modularizing (biomedical) data science. For more details, instructions, and modules check out the project's repository.
> This workflow adheres to the module specifications of [MrBiomics](https://github.com/epigen/MrBiomics), an effort to augment research by modularizing (biomedical) data science. For more details, instructions, and modules check out the project's repository.
>
> ⭐️ **Star and share modules you find valuable** 📤 - help others discover them, and guide our future work!
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cd resources/atacseq_pipeline/mm10
unzip indices_for_Bowtie2.zip && rm indices_for_Bowtie2.zip
```
- Recommended compatible [MR.PARETO](https://github.com/epigen/mr.pareto) modules for downstream analysis (in that order):
- Recommended compatible [MrBiomics](https://github.com/epigen/MrBiomics) modules for downstream analysis (in that order):
- [Genome Browser Track Visualization](https://github.com/epigen/genome_tracks/) for quality control and visual inspection/analysis of genomic regions/genes of interest or top hits.
- [<ins>Sp</ins>lit, F<ins>ilter</ins>, Norma<ins>lize</ins> and <ins>Integrate</ins> Sequencing Data](https://github.com/epigen/spilterlize_integrate/) after count quantification.
- [Unsupervised Analysis](https://github.com/epigen/unsupervised_analysis) to understand and visualize similarities and variations between cells/samples, including dimensionality reduction and cluster analysis. Useful for all tabular data including single-cell and bulk sequencing data.
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