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Ingest to phylogenetic failure during refine #122

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joverlee521 opened this issue Feb 7, 2025 · 3 comments · Fixed by #123
Closed

Ingest to phylogenetic failure during refine #122

joverlee521 opened this issue Feb 7, 2025 · 3 comments · Fixed by #123
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@joverlee521
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Latest automated workflow failed with an error from augur refine

[batch] [2025-02-07T17:18:37+00:00] ERROR: TreeAnc.optimal_branch_length: terminal node alignments required; sequence is missing for leaf: 'A/Geoffroy_sCat/WA/24-037410-004-original/2024'. Missing terminal sequences can be inferred from sister nodes by rerunning with `reconstruct_tip_states=True` or `--reconstruct-tip-states`
[batch] [2025-02-07T17:18:37+00:00] ERROR from TreeTime: This error is most likely due to a problem with your input data.
[batch] [2025-02-07T17:18:37+00:00] Please check your input data and try again. If you continue to have problems, please open a new issue including
[batch] [2025-02-07T17:18:37+00:00] the original command and the error above:  <https://github.com/nextstrain/augur/issues/new/choose>
[batch] [2025-02-07T17:18:37+00:00] augur refine is using TreeTime version 0.11.4
@joverlee521 joverlee521 added the bug Something isn't working label Feb 7, 2025
@joverlee521
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Ah, similar error to #113.
The strain name is changed from A/Geoffroy'sCat/WA/24-037410-004-original/2024 to A/Geoffroy_sCat/WA/24-037410-004-original/2024 by augur tree/iqtree, so it no longer matches the sequence name in the aligned.fasta.

I totally thought I fixed this type of issue in strain names in b6f9b56 🤔

@joverlee521
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I totally thought I fixed this type of issue in strain names in b6f9b56 🤔

OH this was specifically fixed in the Andersen lab script, this new error is a NCBI GenBank record.

@jameshadfield
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The underlying Augur bug is nextstrain/augur#1084

@joverlee521 joverlee521 self-assigned this Feb 7, 2025
joverlee521 added a commit that referenced this issue Feb 7, 2025
Adding latest hosts that I saw while fixing the strain name issue
<#122>
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