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Technical replicates must have different FastQ file names for the pipeline to work. In my case the same pool of libraries was sequenced on two flow cells and had the exact same file names. The pipeline then tries to cat the files together (I think this was the step that failed) but nextflow can't produce the symlinks in the work directory because the multiple files have the same file name.
In the meantime I have changed the file name of the FastQ so that I have _00{1,2}.fastq.gz to distinguish the flow cells on which the libraries were sequenced and the pipeline seems happy with that.
The text was updated successfully, but these errors were encountered:
Description of the bug
Technical replicates must have different FastQ file names for the pipeline to work. In my case the same pool of libraries was sequenced on two flow cells and had the exact same file names. The pipeline then tries to
cat
the files together (I think this was the step that failed) but nextflow can't produce the symlinks in the work directory because the multiple files have the same file name.In the meantime I have changed the file name of the FastQ so that I have
_00{1,2}.fastq.gz
to distinguish the flow cells on which the libraries were sequenced and the pipeline seems happy with that.The text was updated successfully, but these errors were encountered: