Leonardo is a toolbox able to resolve all sample-induced aberrations in selective plane illumination microscopy (SPIM, also called light-sheet fluorescence microscopy, LSFM) by using two major modules: (1) DeStripe removes the stripe artifacts caused by light absorption; (2) FUSE reconstructs one single high-quality image from dual-sided illumination and/or dual-sided detection while eliminating optical distortions (ghosts) caused by light refraction.
https://leonardo-lsfm.readthedocs.io/en/latest/installation.html
Stable Release: pip install leonardo_toolset
Development Head: pip install git+https://github.com/peng-lab/leonardo_toolset.git
Full software including napari plugins: pip install leonardo_toolset[napari]
This leonardo_toolset package contains everything you need for using the toolset, including batch processing support for different workflows, such as running destriping then fusion. If you need only one specific component, you can find more info below:
More details about the leonardo_toolset package
## packages for core componets:
* Leonardo-DeStripe: https://github.com/peng-lab/lsfm_destripe
* Leonardo-FUSE: https://github.com/peng-lab/lsfm_fuse
## packages for napari plugins:
* plugin for Leonardo-DeStripe: https://github.com/peng-lab/lsfm_destripe_napari
* plugin for Leonardo-FUSE: https://github.com/peng-lab/lsfm_fusion_napari
See CONTRIBUTING.md for information related to developing the code.
MIT license