amplicon: Fix a big introduced in v2.7.0. When more than one pairs of…

… primers are given, only the last one is used. #457. introduced in fixing #439

CHANGELOG.md

@@ -1,3 +1,7 @@
+- [SeqKit v2.8.2](https://github.com/shenwei356/seqkit/releases/tag/v2.8.2) - 2024-04-07
+[![Github Releases (by Release)](https://img.shields.io/github/downloads/shenwei356/seqkit/v2.8.2/total.svg)](https://github.com/shenwei356/seqkit/releases/tag/v2.8.2)
+    - `seqkit amplicon`:
+        - Fix a big introduced in v2.7.0. When more than one pairs of primers are given, only the last one is used. [#457](https://github.com/shenwei356/seqkit/issues/457)
 - [SeqKit v2.8.1](https://github.com/shenwei356/seqkit/releases/tag/v2.8.1) - 2024-04-07
 [![Github Releases (by Release)](https://img.shields.io/github/downloads/shenwei356/seqkit/v2.8.1/total.svg)](https://github.com/shenwei356/seqkit/releases/tag/v2.8.1)
     - `seqkit sana`:

seqkit/cmd/amplicon.go

@@ -197,8 +197,14 @@ Examples:
 		// finder pools
 		finderPools := make([]*sync.Pool, len(primers))
 		for i, primer := range primers {
+			f := make([]byte, len(primer[1])) // copy the primer sequence
+			copy(f, primer[1])
+
+			r := make([]byte, len(primer[2])) // copy the primer sequence
+			copy(r, primer[2])
+
 			finderPools[i] = &sync.Pool{New: func() interface{} {
-				finder, _ := NewAmpliconFinder([]byte{'A'}, primer[1], primer[2], maxMismatch)
+				finder, _ := NewAmpliconFinder([]byte{'A'}, f, r, maxMismatch)
 				return finder
 			}}
 		}